Transcripts of Calcium/Calmodulin-dependent Kinases are Changed After Forskolin- or IBMX-induced Insulin Secretion due to Melatonin Treatment of Rat Insulinoma ?-Cells (INS-1)*

The objective of the present study was to examine the effects of melatonin on transcripts of isoforms of calcium/calmodulin-dependent protein kinases in rat insulinoma ?-cells INS-1. Investigations show that calcium/calmodulin-dependent kinase IV and calcium/calmodulin-dependent kinase 2d are expressed in human and rat pancreatic islets and INS-1 cells. By application of either forskolin or 3-isobutyl-1-methylxanthine for 6 hours, calcium spiking was evoked and the release of insulin was increased. The expression of the calcium/calmodulin-dependent kinase IV and calcium/calmodulin-dependent kinase 2d transcripts was significantly increased due to forskolin or 3-isobutyl-1-methylxanthine. Acute melatonin treatment (6?h) in the presence of either forskolin or 3-isobutyl-1-methylxa…

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