Melatonin receptor 1-dependent gene expression in the mouse pars tuberalis as revealed by cDNA microarray analysis and in situ hybridization

In this study, microarray analysis was performed to screen for genes coding for transcriptional regulators under the control of MT1 receptors in the mouse PT. Gene expression levels were compared between melatonin-proficient mice deficient for MT1 (MT1[minus]/[minus]) and the corresponding wild-type (WT) during mid-subjective day (CT06, low endogenous melatonin levels) and mid-subjective night (CT18, high endogenous melatonin levels). In situ hybridization was used to validate the data obtained by microarray analysis to analyze the acute effect of daytime melatonin application on gene expression in the wild-type PT. In the wild-type PT, expression of Tim was higher during day as compared to night. These day/night differences in gene expression were not observed in the PT of MT1[minus]/[min…

Related posts:

1. Removal of Melatonin Receptor Type 1 Induces Insulin Resistance in the Mouse.
2. 24-Hour variation in gene expression of redox pathway enzymes in rat hypothalamus: effect of melatonin treatment.
3. Characterization of the melatonin receptor gene MT1 in mouflon (Ovis Gmelini Musimon) and its relationship with reproductive activity
4. Blocking of melatonin synthesis and MT(1) receptor impairs the activation of Jurkat T cells.
5. Differential Regulation of Kiss1 Expression by Melatonin and Gonadal Hormones in Male and Female Syrian Hamsters