Visualization of melatonin’s multiple mitochondrial levels of protection against mitochondrial Ca2+-mediated permeability transition and beyond in rat brain astrocytes

This study, thus, investigated this possibility of an ‘oxidation free Ca2+ stress’ in the presence of vitamin E after ionomycin exposure as a sole Ca2+-mediated MPT in order to exclude melatonin’s primary antioxidative effects as well as Ca2+-mediated oxidative stress. The studies were carried out using cultured rat brain astrocytes RBA-1. With the application of laser scanning multiple fluorescence imaging microscopy, we visualized for the first time multiple mitochondrial protective effects provided by melatonin during Ca2+ stress. First, melatonin, due to its primary antioxidative actions, completely prevented mCa2+-induced mROS formation during ionomycin exposure. Secondly, when melatonin’s antioxidative effects were prevented due to the addition of vitamin E, melatonin significantly p…

Related posts:

1. T.P.5.02 Melatonin prevents oxidative-stress mediated mitochondrial permeability transition and death in mouse skeletal muscle cells
2. Melatonin prevents oxidative stress-mediated mitochondrial permeability transition and death in skeletal muscle cells
3. Melatonin protects against heart ischemia-reperfusion injury by inhibiting mitochondrial permeability transition pore opening
4. Cellular and Vascular Changes in the Retina of Neonatal Rats after an Acute Exposure to Hypoxia [Retina]
5. Chronic melatonin treatment and its precursor L-tryptophan improve the monoaminergic neurotransmission and related behavior in the aged rat brain