Melatonin reverses apoptosis through which menadione inhibits intestinal calcium absorption

We have previously demonstrated that menadione (MEN) inhibits intestinal calcium (Ca2+) absorption through the mitochondria apoptotic pathway (BBA, 2008). Afterwards, we have observed that this inhibition could be reversed by melatonin (MEL) administration. The aim of this study was to elucidate the molecular mechanisms of this protection caused by MEL. Four-week-old chicks were divided in four groups: 1) controls, 2) treated i.p. with 2.5 ?mol MEN/kg of b.w., 3) treated i.p. with 10 mg MEL/kg of b.w., and 4) treated i.p. with MEL after i.p. MEN administration. Glutathione (GSH) content and the activities of superoxide dismutase (SOD), catalase (CAT), and caspase 3 were measured by spectrophotometric methods. Apoptosis was evaluated by the TUNEL technique and cytochrome c localization. …

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