T.P.5.02 Melatonin prevents oxidative-stress mediated mitochondrial permeability transition and death in mouse skeletal muscle cells

In this study, we demonstrate that melatonin, the main hormone of the pineal gland, readily rescued mitochondria from oxidative stress-induced dysfunction and effectively prevented subsequent apoptosis in C57BL/6J myotubes. In particular, melatonin potently prevented myotube death induced by tert-butylhydroperoxide (t-BHP) in a concentration-dependent manner (10?4–10?6M). Moreover, melatonin maintained plasma membrane integrity after t-BHP exposure and prevented t-BHP-induced fissions of the long mitochondrial filaments and mitochondrial swelling. To determine if the mitochondrial protection provided by melatonin was due to the inhibition of the formation of reactive oxygen species (ROS), intracellular ROS levels were measured using fluorescence imaging. Application of t-BHP produced…

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